| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Claudin 16 |
| Specificity: |
This antibody reacts specifically with Claudin 16 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Claudin 16 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Mutations in PCLN-1 cause renal magnesium wasting, PCLN-1 contains four transmembrane domains and intracellular amino and carboxy termini, PCLN-1 forms an intercellular pore and controls the resorption of magnesium and calcium in the thick ascending limb of Henle (TAL), The human gene PCLN-1 (paracellin-1) is related to the claudin family of integral membrane proteins, They form close connections to eliminate the extracellular space and regulate the flow of solutes between cells, and is detected only at the tight junctions of kidney tissue, characteristic of the other claudin family members, renal hypomagnesemia with hypercalciuria and nephrocalcinosis, which localize to tight junctions, which may contribute to a rare autosomal recessive disease, Tight junctions mediate the regulation of the paracellular pathway between epithelial and endothelial cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PCLN1 HOMG3 |
| Also known as: |
Claudin 16 Polyclonal Antibody |
| Other name: |
Anti-Claudin 16 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Claudin 16 |
| Short name: |
Anti-Claudin 16 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Claudin 16 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2037 |
| Gene: |
CLDN16 |
More about : CLDN16 |
| Long gene name: |
claudin 16 |
| Synonyms name: |
paracellin-1 hypomagnesemia 3, with hypercalciuria and nephrocalcinosis |
| Locus: |
3q28 |
| Discovery year: |
2000-01-07 |
| GenBank acession: |
AF152101 |
| Entrez gene record: |
10686 |
| Pubmed identfication: |
10390358 |
| RefSeq identity: |
NM_006580 |
| Classification: |
Claudins |
| Havana BLAST/BLAT: |
OTTHUMG00000156215 |