| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SMC1 |
| Specificity: |
This antibody reacts specifically with SMC1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SMC1 |
| Gene ID: |
8243 |
| Swiss Prot: |
Q14683 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
At anaphase, Central component of cohesin complex, Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped, The cohesin complex is required for the cohesion of sister chromatids after DNA replication, The cohesin complex may also play a role in spindle pole assembly during mitosis, Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint, allowing sister chromatids to segregate, or via its phosphorylation by ATR, the complex is cleaved and dissociates from chromatin, Involved in chromosome cohesion during cell cycle and in DNA repair |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
bK268H5 |
| Also known as: |
SMC1 Polyclonal Antibody |
| Other name: |
Anti-SMC1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SMC1 |
| Short name: |
Anti-SMC1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SMC1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11112 |
| Gene: |
SMC1B |
More about : SMC1B |
| Long gene name: |
structural maintenance of chromosomes 1B |
| Synonyms gene: |
SMC1L2 |
| Synonyms gene name: |
SMC1 (structural maintenance of chromosomes 1, yeast)-like 1 SMC1 structural maintenance of chromosomes 1-like 2 (yeast) |
| Locus: |
22q13, 31 |
| Discovery year: |
2000-01-12 |
| GenBank acession: |
AJ504806 |
| Entrez gene record: |
27127 |
| Pubmed identfication: |
10591208 11564881 |
| RefSeq identity: |
NM_148674 |
| Classification: |
Structural maintenance of chromosomes proteins Cohesin complex |
| Havana BLAST/BLAT: |
OTTHUMG00000151334 |