| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SMC1 Ser360 |
| Specificity: |
This antibody reacts specifically with SMC1 (Ser360) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser360 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human SMC1 around the phosphorylation site of Ser360 |
| Gene ID: |
8243 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A number of cellular factors interact with hSMC1/hSMC3 during cell cycle, A subset of hSMC1/hSMC3 is localized to spindle poles, DNA recombination and repair and global transcriptional repression, Human cohesion complex associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition, In addition, SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells, Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase, The chromosome proteins are conserved in eukaryotes and can lead to mitotic chromosome segregation defects, The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase, hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo, including mitotic chromosome organization, spindles and kinetochores during mitosis when cohesin is in the cytoplasm, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics, Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SMC 1, SMC1, SMC1A, SMC1A_HUMAN, SMC1B, SMC1BETA, SMC1L1, SMC1L2, SMC1alpha, SMC1alpha protein, SMC1beta protein, SMCB, Segregation of mitotic chromosomes 1, Segregation of mitotic chromosomes like 1, Structural Maintenance of Chromosomes-1 Like 1, Structural maintenance of chromosome 1 like 1 protein, Structural maintenance of chromosome 1 like 2 protein, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes 1B, structural maintenance of chromosomes 1-like 2 yeast, CDLS2 |
| Also known as: |
SMC1 (Ser360) Polyclonal Antibody |
| Other name: |
Anti-SMC1 (Ser360) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SMC1 (Ser360) |
| Short name: |
Anti-SMC1 (Ser360) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SMC1 (Ser360) (polyclonal) |
| Alternative technique: |
polyclonals |