| Also known as: |
TIM-2 |
| Other names: |
T cell immunoglobulin and mucin domain containing 2, T-cell immunoglobulin and mucin domain containing 2, T-cell immunoglobulin and mucin domain-containing protein 2, T-cell immunoglobulin and mucin domain-containing protein 2, T-cell membrane protein 2, T-cell membrane protein 2, TIM-2, TIMD-2, T-cell immunoglobulin and mucin domain-containing protein 2 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
TIM-2 |
| Other gene names: |
AA816106, AU019358, AU019662, C78111, G9D3, TIM-2, TIMD-2, Tim2, Tim2, Timd2, Timd2 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
RMT2-25 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Tim-2 also acts as a receptor for Semaphorin 4A, _x000D__x000D_Studies using clone RMT2-25 in mouse, _x000D__x000D_Tim-2 acts as a negative regulator of Th2 cells, a transmembrane glycoprotein and member of the immunoglobulin superfamily, a transmembrane protein expressed on dendritic cells and B cells, and has been shown to enhance T cell activation, and is implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses, and preferentially up-regulated in Th2 cells and down-regulated in Th1 cells, developmentally in the 8-cell embryo and blastocyst, during early phase CIA (collagen-induced arthritis) _x000D__x000D_Clone RMT2-25 has been shown to significantly enhance the proliferation of stimulated splenic B cells <, expressed by activated lymphocytes, have shown that signaling by Tim-2 can influence the proliferation and antibody production of B cells, which plays a role in the guidance of axonal migration during neuronal development, /i>, TIM-2 Clone RMT2-25 specifically recognises mouse Tim-2 (T-cell immunoglobulin mucin 2), i>, in vitro< |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
TIM-2: |
| Short name: |
RAT ANTI MOUSE TIM-2:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
RAT antibody to MOUSE TIM-2:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |