| Long name: |
FITC Conjugated, KMT1E/SETDB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KMT1E/SETDB1 |
| Specificity: |
This is a highly specific antibody against KMT1E/SETDB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
210-260/1291 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human SETDB1 |
| Gene ID number: |
9869 |
| Swiss Prot: |
Q15047 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306), Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306), CBX3 and/or CBX5) proteins to methylated histones, H3 'Lys-9' trimethylation is coordinated with DNA methylation, H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1, Mainly functions in euchromatin regions, Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation, Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306), SETDB1 is targeted to histone H3 by TRIM28/TIF1B, The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610), a factor recruited by KRAB zinc-finger proteins, thereby playing a central role in the silencing of euchromatic genes, Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
KG1T KIAA0067 ESET KMT1E TDRD21 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KMT1E/SETDB1 Conjugated |
| Short name: |
FITC Conjugated, KMT1E/SETDB1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, KMT1E/SETDB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10761 |
| Gene: |
SETDB1 |
More about : SETDB1 |
| Long gene name: |
SET domain bifurcated 1 |
| Synonyms gene name: |
SET domain, bifurcated 1 |
| Synonyms name: |
tudor domain containing 21 |
| Locus: |
1q21, 3 |
| Discovery year: |
1999-07-19 |
| GenBank acession: |
D31891 |
| Entrez gene record: |
9869 |
| Pubmed identfication: |
10343109 |
| RefSeq identity: |
NM_001145415 |
| Classification: |
Methyl-CpG binding domain containing Lysine methyltransferases Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000035003 |