| Long name: |
Cy5 Conjugated, KMT1E/SETDB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KMT1E/SETDB1 |
| Specificity: |
This is a highly specific antibody against KMT1E/SETDB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
210-260/1291 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human SETDB1 |
| Gene ID number: |
9869 |
| Swiss Prot: |
Q15047 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306), Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306), CBX3 and/or CBX5) proteins to methylated histones, H3 'Lys-9' trimethylation is coordinated with DNA methylation, H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1, Mainly functions in euchromatin regions, Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation, Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306), SETDB1 is targeted to histone H3 by TRIM28/TIF1B, The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610), a factor recruited by KRAB zinc-finger proteins, thereby playing a central role in the silencing of euchromatic genes, Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
625, 650nm/670nm |
| Synonyms: |
KG1T KIAA0067 ESET KMT1E TDRD21 |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KMT1E/SETDB1 Conjugated |
| Short name: |
Conjugated, KMT1E/SETDB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy5 |
| Alternative name: |
cyanine 5 coupled, KMT1E/SETDB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10761 |
| Gene: |
SETDB1 |
More about : SETDB1 |
| Long gene name: |
SET domain bifurcated 1 |
| Synonyms gene name: |
SET domain, bifurcated 1 |
| Synonyms name: |
tudor domain containing 21 |
| Locus: |
1q21, 3 |
| Discovery year: |
1999-07-19 |
| GenBank acession: |
D31891 |
| Entrez gene record: |
9869 |
| Pubmed identfication: |
10343109 |
| RefSeq identity: |
NM_001145415 |
| Classification: |
Methyl-CpG binding domain containing Lysine methyltransferases Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000035003 |