| Long name: |
ALEXA FLUOR 647 Conjugated, KMT1E/SETDB1 Polyclonal Antibody |
| Also known as: |
Anti-KMT1E/SETDB1 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KMT1E/SETDB1 |
| Specificity: |
This is a highly specific antibody against KMT1E/SETDB1 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
210-260/1291 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SETDB1 |
| Gene ID Number: |
9869 |
| Swiss Prot: |
Q15047 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306), Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306), CBX3 and/or CBX5) proteins to methylated histones, H3 'Lys-9' trimethylation is coordinated with DNA methylation, H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1, Mainly functions in euchromatin regions, Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation, Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306), SETDB1 is targeted to histone H3 by TRIM28/TIF1B, The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610), a factor recruited by KRAB zinc-finger proteins, thereby playing a central role in the silencing of euchromatic genes, Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
KG1T KIAA0067 ESET KMT1E TDRD21 |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the KMT1E/SETDB1 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
KMT1E/SETDB1 |
| Short name: |
KMT1E/SETDB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, KMT1E/SETDB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10761 |
| Gene: |
SETDB1 |
More about : SETDB1 |
| Long gene name: |
SET domain bifurcated 1 |
| Synonyms gene name: |
SET domain, bifurcated 1 |
| Synonyms name: |
tudor domain containing 21 |
| Locus: |
1q21, 3 |
| Discovery year: |
1999-07-19 |
| GenBank acession: |
D31891 |
| Entrez gene record: |
9869 |
| Pubmed identfication: |
10343109 |
| RefSeq identity: |
NM_001145415 |
| Classification: |
Methyl-CpG binding domain containing Lysine methyltransferases Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000035003 |