| Long name: |
Biotin Conjugated, KMT1E/SETDB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KMT1E/SETDB1 |
| Specificity: |
This is a highly specific antibody against KMT1E/SETDB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
210-260/1291 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human SETDB1 |
| Gene ID number: |
9869 |
| Swiss Prot: |
Q15047 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306), Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306), CBX3 and/or CBX5) proteins to methylated histones, H3 'Lys-9' trimethylation is coordinated with DNA methylation, H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1, Mainly functions in euchromatin regions, Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation, Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306), SETDB1 is targeted to histone H3 by TRIM28/TIF1B, The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610), a factor recruited by KRAB zinc-finger proteins, thereby playing a central role in the silencing of euchromatic genes, Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
KG1T KIAA0067 ESET KMT1E TDRD21 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KMT1E/SETDB1 Conjugated |
| Short name: |
Conjugated, KMT1E/SETDB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, KMT1E/SETDB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10761 |
| Gene: |
SETDB1 |
More about : SETDB1 |
| Long gene name: |
SET domain bifurcated 1 |
| Synonyms gene name: |
SET domain, bifurcated 1 |
| Synonyms name: |
tudor domain containing 21 |
| Locus: |
1q21, 3 |
| Discovery year: |
1999-07-19 |
| GenBank acession: |
D31891 |
| Entrez gene record: |
9869 |
| Pubmed identfication: |
10343109 |
| RefSeq identity: |
NM_001145415 |
| Classification: |
Methyl-CpG binding domain containing Lysine methyltransferases Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000035003 |