| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
KMT1E/SETDB1/ESET |
| Specificity: |
This antibody reacts specifically with KMT1E/SETDB1/ESET |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human KMT1E |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ESET is a histone methyltransferase, ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin, ESET is widely expressed with highest levels found in testis, It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modulation, SETDB1, The gene encoding for this protein, This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones, maps to chromosome 1q21, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity, ESET is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KG1T KIAA0067 ESET KMT1E TDRD21 |
| Also known as: |
KMT1E/SETDB1/ESET Polyclonal Antibody |
| Other name: |
Anti-KMT1E/SETDB1/ESET Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
KMT1E/SETDB1/ESET |
| Short name: |
Anti-KMT1E/SETDB1/ESET (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-KMT1E/SETDB1/ESET (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10761 |
| Gene: |
SETDB1 |
More about : SETDB1 |
| Long gene name: |
SET domain bifurcated 1 |
| Synonyms gene name: |
SET domain, bifurcated 1 |
| Synonyms name: |
tudor domain containing 21 |
| Locus: |
1q21, 3 |
| Discovery year: |
1999-07-19 |
| GenBank acession: |
D31891 |
| Entrez gene record: |
9869 |
| Pubmed identfication: |
10343109 |
| RefSeq identity: |
NM_001145415 |
| Classification: |
Methyl-CpG binding domain containing Lysine methyltransferases Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000035003 |