| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MLCK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the MLCK antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This MLCK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q15746 |
| Purity: |
Purified |
| Description: |
In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells, It is a central determinant in the development of vascular permeability and tissue edema formation, MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis, MLCK is probably down-regulated by phosphorylation, Non-muscle isoform 2 is the dominant splice variant expressed in various tissues, Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific, The Telokin isoform, The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains, a member of the Ser/Thr protein kinase family, an event that facilitates myosin interaction with actin filaments, has been found in a wide variety of adult and fetal tissues, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), which binds calmodulin, MLCK |
| Immunogen: |
A portion of amino acids 908-938 from the human protein was used as the immunogen for this MLCK antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the MLCK antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MLCK |
| Short name: |
MLCK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MLCK (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
29826 |
| Gene: |
MYLK3 |
More about : MYLK3 |
| Long gene name: |
myosin light chain kinase 3 |
| Synonyms: |
caMLCK MLCK |
| Synonyms name: |
MLC kinase |
| Locus: |
16q11, 2 |
| Discovery year: |
2008-01-23 |
| GenBank acession: |
AJ247087 |
| Entrez gene record: |
91807 |
| Pubmed identfication: |
17885681 |
| RefSeq identity: |
NM_182493 |
| Havana BLAST/BLAT: |
OTTHUMG00000132543 |