| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MLCK |
| Specificity: |
This antibody reacts specifically with MLCK |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MLCK |
| Gene ID: |
4638 |
| Swiss Prot: |
Q15746 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A pseudogene is located on the p arm of chromosome 3, Additional variants have been identified but lack full length transcripts, Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin, In addition, Jul 2008], This gene encodes both smooth muscle and nonmuscle isoforms, This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity, [provided by RefSeq, a muscle member of the immunoglobulin gene superfamily, a small protein identical in sequence to the C-terminus of myosin light chain kinase, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme, it encodes telokin, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments, using a separate promoter in an intron in the 3' region, This gene |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
caMLCK MLCK |
| Also known as: |
MLCK Polyclonal Antibody |
| Other name: |
Anti-MLCK Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MLCK |
| Short name: |
Anti-MLCK (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MLCK (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
29826 |
| Gene: |
MYLK3 |
More about : MYLK3 |
| Long gene name: |
myosin light chain kinase 3 |
| Synonyms name: |
MLC kinase |
| Locus: |
16q11, 2 |
| Discovery year: |
2008-01-23 |
| GenBank acession: |
AJ247087 |
| Entrez gene record: |
91807 |
| Pubmed identfication: |
17885681 |
| RefSeq identity: |
NM_182493 |
| Havana BLAST/BLAT: |
OTTHUMG00000132543 |