| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MEK4 Thr261 |
| Specificity: |
MAP2K6 when phosphorylated at Thr211, and MAP2K7 when phosphorylated at Thr275, MEK4 (Thr261), This antibody will cross react with MAP2K3 when phosphorylated at Thr222 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr261 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human MEK4 around the phosphorylation site of Thr261 |
| Gene ID: |
6416 |
| Swiss Prot: |
P45985 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, MAP2K4 is required for maintaining peripheral lymphoid homeostasis, MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, MAPK12, MAPK13 and MAPK14, MAPK9/JNK2 and MAPK10/JNK3, The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, Whereas MAP2K7/MKK7 exclusively activates JNKs, With MAP2K7/MKK7, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif, including the release cytochrome c, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, leading to apoptosis, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs, Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MEK4 JNKK1 PRKMK4 MKK4 |
| Also known as: |
MEK4 (Thr261) Polyclonal Antibody |
| Other name: |
Anti-MEK4 (Thr261) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MEK4 (Thr261) |
| Short name: |
Anti-MEK4 (Thr261) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MEK4 (Thr261) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6844 |
| Gene: |
MAP2K4 |
More about : MAP2K4 |
| Long gene name: |
mitogen-activated protein kinase kinase 4 |
| Synonyms gene: |
SERK1 |
| Locus: |
17p12 |
| Discovery year: |
1997-11-11 |
| GenBank acession: |
L36870 |
| Entrez gene record: |
6416 |
| Pubmed identfication: |
7716521 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000178267 |