| Long name: |
Cy5, 5 Conjugated, MEK4 (Thr261) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy5, 5, 5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MEK4 Thr261 |
| Specificity: |
MAP2K6 when phosphorylated at Thr211, This antibody will cross react with MAP2K3 when phosphorylated at Thr222, and MAP2K7 when phosphorylated at Thr275, This is a highly specific antibody against MEK4 Thr261 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr261 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
240-280/399 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MEK4 around the phosphorylation site of Thr261 |
| Gene ID number: |
6416 |
| Swiss Prot: |
P45985 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, MAP2K4 is required for maintaining peripheral lymphoid homeostasis, MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, MAPK12, MAPK13 and MAPK14, MAPK9/JNK2 and MAPK10/JNK3, The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, Whereas MAP2K7/MKK7 exclusively activates JNKs, With MAP2K7/MKK7, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif, including the release cytochrome c, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, leading to apoptosis, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs, Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
675nm/694nm |
| Synonyms: |
MEK4 JNKK1 PRKMK4 MKK4 |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths |
| Gene target: |
MEK4 (Thr261) Conjugated |
| Short name: |
Conjugated, MEK4 (Thr261) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
5, Cy5 |
| Alternative name: |
cyanine 5, 5 coupled, MEK4 (Thr261) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6844 |
| Gene: |
MAP2K4 |
More about : MAP2K4 |
| Long gene name: |
mitogen-activated protein kinase kinase 4 |
| Synonyms gene: |
SERK1 |
| Locus: |
17p12 |
| Discovery year: |
1997-11-11 |
| GenBank acession: |
L36870 |
| Entrez gene record: |
6416 |
| Pubmed identfication: |
7716521 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000178267 |