| Long name: |
Biotin Conjugated, MEK4 (Thr261) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MEK4 Thr261 |
| Specificity: |
MAP2K6 when phosphorylated at Thr211, This antibody will cross react with MAP2K3 when phosphorylated at Thr222, and MAP2K7 when phosphorylated at Thr275, This is a highly specific antibody against MEK4 Thr261 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr261 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
240-280/399 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MEK4 around the phosphorylation site of Thr261 |
| Gene ID number: |
6416 |
| Swiss Prot: |
P45985 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, MAP2K4 is required for maintaining peripheral lymphoid homeostasis, MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, MAPK12, MAPK13 and MAPK14, MAPK9/JNK2 and MAPK10/JNK3, The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, Whereas MAP2K7/MKK7 exclusively activates JNKs, With MAP2K7/MKK7, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif, including the release cytochrome c, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, leading to apoptosis, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs, Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
MEK4 JNKK1 PRKMK4 MKK4 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MEK4 (Thr261) Conjugated |
| Short name: |
Conjugated, MEK4 (Thr261) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, MEK4 (Thr261) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6844 |
| Gene: |
MAP2K4 |
More about : MAP2K4 |
| Long gene name: |
mitogen-activated protein kinase kinase 4 |
| Synonyms gene: |
SERK1 |
| Locus: |
17p12 |
| Discovery year: |
1997-11-11 |
| GenBank acession: |
L36870 |
| Entrez gene record: |
6416 |
| Pubmed identfication: |
7716521 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000178267 |