| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PLK1 Ser137 |
| Specificity: |
PLK1 (Ser137), This phosphorylation site is homologous across the listed species |
| Modification: |
Phosphorylation |
| Modification site: |
Ser137 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137 |
| Gene ID: |
5347 |
| Swiss Prot: |
P53350 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Contributes to the regulation of AURKA function, NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, Phosphorylates BORA, Phosphorylates NEDD1, Phosphorylates SGOL1, Phosphorylates both ECT2 and RACGAP1, Promotes the central spindle recruitment of ECT2, Regulates TP53 stability through phosphorylation of TOPORS, Required for kinetochore localization of BUB1B, Required for recovery after DNA damage checkpoint and entry into mitosis, Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function, an important step for spindle formation, and the regulation of mitotic exit and cytokinesis, and thereby promotes the degradation of BORA, and thereby stimulates their interaction that is essential for the cleavage furrow formation, including the regulation of centrosome maturation and spindle assembly, the inactivation of APC/C inhibitors, the removal of cohesins from chromosome arms, Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
PLK1 (Ser137) Polyclonal Antibody |
| Other name: |
Anti-PLK1 (Ser137) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PLK1 (Ser137) |
| Short name: |
Anti-PLK1 (Ser137) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PLK1 (Ser137) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9077 |
| Gene: |
PLK1 |
More about : PLK1 |
| Long gene name: |
polo like kinase 1 |
| Synonyms gene: |
PLK |
| Synonyms gene name: |
polo-like kinase (Drosophila) polo-like kinase 1 |
| Locus: |
16p12, 2 |
| Discovery year: |
1994-07-25 |
| Entrez gene record: |
5347 |
| Pubmed identfication: |
8127874 |
| RefSeq identity: |
NM_005030 |
| Havana BLAST/BLAT: |
OTTHUMG00000096984 |