| Long name: |
Cy3 Conjugated, PLK1 (Ser137) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PLK1 Ser137 |
| Specificity: |
This phosphorylation site is homologous across the listed species, This is a highly specific antibody against PLK1 Ser137 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser137 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
110-160/603 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137 |
| Gene ID number: |
5347 |
| Swiss Prot: |
P53350 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Contributes to the regulation of AURKA function, NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, Phosphorylates BORA, Phosphorylates NEDD1, Phosphorylates SGOL1, Phosphorylates both ECT2 and RACGAP1, Promotes the central spindle recruitment of ECT2, Regulates TP53 stability through phosphorylation of TOPORS, Required for kinetochore localization of BUB1B, Required for recovery after DNA damage checkpoint and entry into mitosis, Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function, an important step for spindle formation, and the regulation of mitotic exit and cytokinesis, and thereby promotes the degradation of BORA, and thereby stimulates their interaction that is essential for the cleavage furrow formation, including the regulation of centrosome maturation and spindle assembly, the inactivation of APC/C inhibitors, the removal of cohesins from chromosome arms, Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Properties: |
1 of the PLK1 (Ser137) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PLK1 (Ser137) Conjugated |
| Short name: |
Conjugated, PLK1 (Ser137) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, PLK1 (Ser137) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9077 |
| Gene: |
PLK1 |
More about : PLK1 |
| Long gene name: |
polo like kinase 1 |
| Synonyms gene: |
PLK |
| Synonyms gene name: |
polo-like kinase (Drosophila) polo-like kinase 1 |
| Locus: |
16p12, 2 |
| Discovery year: |
1994-07-25 |
| Entrez gene record: |
5347 |
| Pubmed identfication: |
8127874 |
| RefSeq identity: |
NM_005030 |
| Havana BLAST/BLAT: |
OTTHUMG00000096984 |