| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
14-3-3 gamma |
| Specificity: |
This antibody reacts specifically with 14-3-3 gamma |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human 14-3-3 gamma |
| Gene ID: |
7532 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
14-3-3 proteins form dimers that present two binding sites for ligand proteins, 14-3-3 proteins function in broad regulation of these ligand proteins, Loss of expression contributes to a vast array of pathogenic cellular activities, Seven isoforms comprise this family of signaling intermediates, These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation, activation/repression of enzymatic activity, and facilitation of protein modification, by cytoplasmic sequestration, denoted 14-3-3 b, e, g, h, mitogenic signaling and cell-cycle checkpoints, notably apoptosis, occupation of interaction domains and import/export sequences, prevention of degradation, q and s, thereby bringing together two proteins that may not otherwise associate, z, 14-3-3 proteins regulate many cellular processes relevant to cancer biology |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HS1 14-3-3 |
| Also known as: |
14-3-3 gamma Polyclonal Antibody |
| Other name: |
Anti-14-3-3 gamma Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
14-3-3 gamma |
| Short name: |
Anti-14-3-3 gamma (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-14-3-3 g (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12854 |
| Gene: |
YWHAQ |
More about : YWHAQ |
| Long gene name: |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| Synonyms gene name: |
theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein |
| Synonyms name: |
protein tau |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-08-20 |
| GenBank acession: |
AF070556 |
| Entrez gene record: |
10971 |
| Pubmed identfication: |
11080204 |
| RefSeq identity: |
NM_006826 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000013848 |