| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
AMPK gamma 1/2/3 |
| Specificity: |
This antibody reacts specifically with AMPK gamma 1/2/3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm, Nucleus, Extracellular |
| Antigen Source: |
³, 2, KLH conjugated synthetic peptide derived from human PRKAG2/AMPKÎ |
| Gene ID: |
5571, 51422, 53632 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein, Human AMPKg2 and AMPKg3, It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways, The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, The human AMPKg1 gene encodes a 331 amino acid protein, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation, respectively, respectively, respectively, which are 569 and 492 amino acid proteins, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, AMPK is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AMPK AMPKa2 |
| Also known as: |
AMPK gamma 1/2/3 Polyclonal Antibody |
| Other name: |
Anti-AMPK gamma 1/2/3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
AMPK gamma 1/2/3 |
| Short name: |
Anti-AMPK gamma 1/2/3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-AMPK g 1/2/3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |