| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
AMPK gamma 3/PRKAG3 |
| Specificity: |
This antibody reacts specifically with AMPK gamma 3/PRKAG3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human AMPK gamma 3 |
| Gene ID: |
53632 |
| Swiss Prot: |
Q9UGI9 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ADP also stimulates phosphorylation, ADP and ATP, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, ATP promotes dephosphorylation of catalytic subunit, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Gamma non-catalytic subunit mediates binding to AMP, In response to reduction of intracellular ATP levels, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits, probably by indirectly activating myosin, rendering the AMPK enzyme inactive, without stimulating already phosphorylated catalytic subunit, AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
AMPK gamma 3/PRKAG3 Polyclonal Antibody |
| Other name: |
Anti-AMPK gamma 3/PRKAG3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
AMPK gamma 3/PRKAG3 |
| Short name: |
Anti-AMPK gamma 3/PRKAG3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, AMP-activated, g 3 non-catalytic subunit (polyclonal), antibody to-AMPK g 3/protein phosphorylation catalyst |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
AMP-activated, Extracellular, PRKAG3 and IDBG-643891 and ENSBTAG00000013492 and 511961, PRKAG3 and IDBG-81160 and ENSG00000115592 and 53632, Prkag3 and IDBG-168633 and ENSMUSG00000006542 and 241113, adenyl nucleotide binding, gamma 3 non-catalytic subunit, this GO :0004679 and AMP-activated protein kinase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005978 and glycogen biosynthetic process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006633 and fatty acid biosynthetic process and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0015758 and glucose transport and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030554 and adenyl nucleotide binding and molecular function this GO :0031588 and AMP-activated protein kinase complex and cellular component this GO :0035556 and intracellular signal transduction and biological process this GO :0061024 and membrane organization and biological process, this GO :0004679 : AMP-activated protein kinase activity, this GO :0004679 : AMP-activated protein kinase activity and also this GO :0005524 : ATP binding and also this GO :0016301 : kinase activity and also this GO :0019901 : protein kinase binding and also this GO :0030554 : adenyl nucleotide binding, this GO :0005524 : ATP binding, this GO :0016301 : kinase activity, this GO :0019901 : protein kinase binding, this GO :0030554 : adenyl nucleotide binding, protein kinase |
| Identity: |
9387 |
| Gene: |
PRKAG3 |
More about : PRKAG3 |
| Long gene name: |
protein kinase AMP-activated non-catalytic subunit gamma 3 |
| Synonyms gene name: |
AMP-activated, gamma 3 non-catalytic subunit , protein kinase |
| Locus: |
2q35 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AF214519 |
| Entrez gene record: |
53632 |
| Pubmed identfication: |
10818001 |
| Havana BLAST/BLAT: |
OTTHUMG00000133078 |