| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
YB1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the YB1 antibody should be determined by the researcher |
| Intented use: |
This YB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P67809 |
| Purity: |
Antigen affinity |
| Description: |
It has a molecular mass of 35, Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, The YBX1 gene is mapped on 1p34, YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box, YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation, Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages, Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, and induced megakaryocytic differentiation in response to arsenic trioxide treatment, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation, commonly referred to as YB-1 by researchers, induced apoptosis, is a human protein, 2, 414 and contains 18% basic residues and putative nuclear localization signals, YBX1 (Y box binding protein 1) |
| Immunogen: |
Amino acids ENPKPQDGKETKAADPPAENSSAPEAEQ of human YB1 were used as the immunogen for the YB1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the YB1 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Nuclear and cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
YB1 |
| Short name: |
YB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
YB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8014 |
| Gene: |
YBX1 |
More about : YBX1 |
| Long gene name: |
Y-box binding protein 1 |
| Synonyms gene: |
NSEP1 |
| Synonyms gene name: |
nuclease sensitive element binding protein 1 |
| Synonyms: |
YB-1 YB1 DBPB NSEP-1 MDR-NF1 BP-8 CSDB CSDA2 |
| Locus: |
1p34, 2 |
| Discovery year: |
1993-10-20 |
| GenBank acession: |
BC013838 |
| Entrez gene record: |
4904 |
| Pubmed identfication: |
1891370 3174636 |
| RefSeq identity: |
NM_004559 |
| Classification: |
Y box binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000007523 |