| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
YB1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the YB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This YB1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P67809 |
| Purity: |
Antigen affinity |
| Description: |
It has a molecular mass of 35, Knockdown of the protein in a human leukemic cell line inhibited proliferation ability, The protein is highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), YBX1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box, YBX1 is downregulated during myeloid differentiation and aberrant expression in leukemic cells may contribute to leukemia development by blocking differentiation, YBX1 is expressed at high levels in myeloid leukemic cells at different developmental stages, a hematopoietic precursor cell line, and induced megakaryocytic differentiation in response to arsenic trioxide treatment, but is downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation, commonly referred to as YBX1 or just YB1 by researchers, induced apoptosis, is a human protein, 414D and contains 18% basic residues and putative nuclear localization signals, Y box binding protein 1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human YBX1 (KYAADRNHYRRYPRRR) was used as the immunogen for this YB1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the YB1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
YB1 |
| Short name: |
YB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
YB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8014 |
| Gene: |
YBX1 |
More about : YBX1 |
| Long gene name: |
Y-box binding protein 1 |
| Synonyms gene: |
NSEP1 |
| Synonyms gene name: |
nuclease sensitive element binding protein 1 |
| Synonyms: |
YB-1 YB1 DBPB NSEP-1 MDR-NF1 BP-8 CSDB CSDA2 |
| Locus: |
1p34, 2 |
| Discovery year: |
1993-10-20 |
| GenBank acession: |
BC013838 |
| Entrez gene record: |
4904 |
| Pubmed identfication: |
1891370 3174636 |
| RefSeq identity: |
NM_004559 |
| Classification: |
Y box binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000007523 |