| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
AIM2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the AIM2 antibody should be determined by the researcher |
| Intented use: |
This AIM2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O14862 |
| Purity: |
Antigen affinity |
| Description: |
AIM2 is a member of the Ifi202/IFI16 family, ASC also contains a CARD domain (caspase activation and recruitment domain), Interferon-gamma induces expression of AIM2, It plays a putative role in tumorigenic reversion and may control cell proliferation, The C-terminal HIN domain binds double stranded DNA (either viral, The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain), This leads to the autoactivation of caspase-1, This leads to the oligomerization of the inflammasome complex, Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18), bacterial, or even host) and acts as a cytosolic dsDNA sensor, that recruits procaspase-1 to the complex, Absent In Melanoma 2 is a protein that in humans is encoded by the AIM2 gene |
| Immunogen: |
Amino acids 14-215 of human AIM2 were used as the immunogen for the AIM2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the AIM2 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
membrane, nuclear, Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
AIM2 |
| Short name: |
AIM2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
AIM2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
357 |
| Gene: |
AIM2 |
More about : AIM2 |
| Long gene name: |
absent in melanoma 2 |
| Synonyms: |
PYHIN4 |
| Locus: |
1q23, 1-q23, 2 |
| Discovery year: |
1999-04-22 |
| GenBank acession: |
AF024714 |
| Entrez gene record: |
9447 |
| Pubmed identfication: |
9242382 |
| RefSeq identity: |
NM_004833 |
| Classification: |
Pyrin and HIN domain family |
| Havana BLAST/BLAT: |
OTTHUMG00000037183 |