| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NLRP3 (NALP3) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the NLRP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This NLRP3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q96P20 |
| Purity: |
Antigen affinity |
| Description: |
Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells, CRYOPYRIN, Caspase-1 activation, Consistent with an essential role for NLRP3 inflammasomes in antifungal immunity, Gross et al, IL-1b production, Mutation of the proteins nucleotide-binding domain reduced ATP binding, NALP3 or PYPAF1, NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC), The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance, The encoded protein may play a role in the regulation of inflammation and apoptosis, The gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes, albicans infection, also known as CIAS1, and association with ASC, cell death, is a protein that in humans is encoded by the NLRP3 gene, macromolecular complex formation, self-association, NOD-like receptor family pyrin domain containing 3, showed that deficient mice are hypersusceptible to C |
| Immunogen: |
An amino acid sequence from the N-terminus of human NLRP3 (RYLEDLEDVDLKKFKMHLED) was used as the immunogen for this NLRP3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NLRP3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NLRP3 (NALP3) |
| Short name: |
NLRP3 Antibody (NALP3) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
pyrin domain containing 3 (Antibody to) (NALP3), NLR family |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cytoplasm, NLRP3 and IDBG-107914 and ENSG00000162711 and 114548, NLRP3 and IDBG-645402 and ENSBTAG00000001273 and 538639, Nlrp3 and IDBG-178924 and ENSMUSG00000032691 and 216799, leucine rich repeat containing receptor signaling pathway and biological process this GO :0042347 and negative regulation of NF-kappaB import into nucleus and biological process this GO :0042834 and peptidoglycan binding and molecular function this GO :0043280 and positive regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0044546 and NLRP3 inflammasome complex assembly and biological process this GO :0045087 and innate immune response and biological process this GO :0050701 and interleukin-1 secretion and biological process this GO :0050713 and negative regulation of interleukin-1 beta secretion and biological process this GO :0050718 and positive regulation of interleukin-1 beta secretion and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051259 and protein oli this GO merization and biological process this GO :0051607 and defense response to virus and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0072559 and NLRP3 inflammasome complex and cellular component, peptidoglycan binding, pyrin domain containing 3, this GO :0002674 and negative regulation of acute inflammatory response and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006952 and defense response and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0009595 and detection of biotic stimulus and biological process this GO :0032088 and negative regulation of NF-kappaB transcription factor activity and biological process this GO :0032611 and interleukin-1 beta production and biological process this GO :0032621 and interleukin-18 production and biological process this GO :0035872 and nucleotide-binding domain, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0042834 : peptidoglycan binding, this GO :0005524 : ATP binding, this GO :0042834 : peptidoglycan binding, NLR family |
| Identity: |
16400 |
| Gene: |
NLRP3 |
More about : NLRP3 |
| Long gene name: |
NLR family pyrin domain containing 3 |
| Synonyms gene: |
C1orf7 CIAS1 |
| Synonyms gene name: |
cold autoinflammatory syndrome 1 |
| Synonyms: |
AGTAVPRL AII AVP FCAS FCU NALP3 PYPAF1 MWS CLR1, 1 |
| Synonyms name: |
Cryopyrin nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3 |
| Locus: |
1q44 |
| Discovery year: |
2001-08-28 |
| GenBank acession: |
AF054176 |
| Entrez gene record: |
114548 |
| Pubmed identfication: |
10741953 |
| RefSeq identity: |
NM_004895 |
| Classification: |
NLR family Pyrin domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000040647 |
| Locus Specific Databases: |
INFEVERS: The repertory of Familial Mediterranean Fever (FMF) and Hereditary Inflammatory Disorders Mutations LRG_197 |