| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
5-Lipoxygenase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the 5-Lipoxygenase antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This 5-Lipoxygenase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P09917 |
| Purity: |
Antigen affinity |
| Description: |
ALOX5 activity is short-lived, Compared with age-matched competent mice, Human ALOX5 contains three nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization, It is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases, Pharmacologic inhibition or gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels, The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes, apparently in part because of an intrinsic instability of the enzyme, the knockout mice developed less right heart hypertrophy, 5-Lipoxygenase is an enzyme that in humans is encoded by the ALOX5 gene |
| Immunogen: |
An amino acid sequence from the C-terminus of human ALOX5 (AERNKKKQLPYYYLSPDR) was used as the immunogen for this 5-Lipoxygenase antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the 5-Lipoxygenase antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
5-Lipoxygenase |
| Short name: |
5-Lipoxygenase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
5-Lipoxygenase (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
435 |
| Gene: |
ALOX5 |
More about : ALOX5 |
| Long gene name: |
arachidonate 5-lipoxygenase |
| Synonyms: |
5-LOX |
| Locus: |
10q11, 21 |
| Discovery year: |
1992-07-10 |
| GenBank acession: |
J03571 |
| Entrez gene record: |
240 |
| Pubmed identfication: |
2565035 |
| Classification: |
Arachidonate lipoxygenases |
| Havana BLAST/BLAT: |
OTTHUMG00000018081 |