| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
12 Lipoxygenase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the 12 Lipoxygenase antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This 12 Lipoxygenase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P18054 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, Expression of the LOG12 gene was detected in human erythroleukemia cells, The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5, The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme, and human umbilical vein endothelial cells by reverse transcription-PCR analysis, is an enzyme that in humans is encoded by the ALOX12 gene, platelets, the ALOX12 gene is located in band 17p13, 1, 14-eicosatetraenoic acid, 8, 10, Arachidonate 12-lipoxygenase |
| Immunogen: |
An amino acid sequence from the C-terminus of human ALOX12 (HHKEKYFSGPKPKAVLNQFR) was used as the immunogen for this 12 Lipoxygenase antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the 12 Lipoxygenase antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
12 Lipoxygenase |
| Short name: |
12 Lipoxygenase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
12 Lipoxygenase (Antibody to) |
| Alternative technique: |
antibodies |