| Long name: |
Cy3 Conjugated, HPV16 E6 protein (2E3) Monoclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Mouse (Mus musculus) |
| Target Antigen: |
HPV16 E6 protein |
| Specificity: |
This is a highly specific antibody against HPV16 E6 protein |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Monoclonal |
| Clone: |
2000 |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
85-158/158 |
| Source: |
KLH conjugated synthetic peptide derived from HPV16 E6 protein |
| Gene ID number: |
1489078 |
| Swiss Prot: |
P03126 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Virus |
| Crossreactive species details: |
HPV16 |
| Antigen background: |
Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development, E6 also inhibits immune response by interacting with host IRF3 and TYK2, E6 associates with host E6-AP ubiquitin-protein ligase, Fas-associated death domain-containing protein (FADD) and procaspase 8, In turn, Other cellular targets including Bak, The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization, These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway, a repressor of human telomerase reverse transcriptase (hTERT), and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation, are degraded by E6/E6AP causing inhibition of apoptosis, Plays a major role in the induction and maintenance of cellular transformation |
| Purification method: |
Purified by Protein G |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
E6, Protein E6 |
| Properties: |
1 of the HPV16 E6 protein (2E3) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HPV16 E6 protein (2E3) Conjugated |
| Short name: |
Conjugated, HPV16 E6 protein (2E3) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, HPV16 E6 protein (2E3) (Antibody to) |
| Alternative technique: |
antibodies |