| Long name: |
HPV16 E6 protein (2E3) Monoclonal Antibody |
| Also known as: |
HPV16 E6 protein (2E3) Monoclonal Antibody |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
HPV16 E6 protein |
| Specificity: |
This is a highly specific antibody against HPV16 E6 protein |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
2000 |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
85-158/158 |
| Source: |
KLH conjugated synthetic peptide derived from HPV16 E6 protein |
| Gene ID number: |
1489078 |
| Swiss Prot: |
P03126 |
| Tested Applications: |
IF(IHC-P), IHC-P |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500) |
| Cross reactivity: |
Virus |
| Cross reactive species details: |
HPV16 |
| Background of the target antigen: |
Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development, E6 also inhibits immune response by interacting with host IRF3 and TYK2, E6 associates with host E6-AP ubiquitin-protein ligase, Fas-associated death domain-containing protein (FADD) and procaspase 8, In turn, Other cellular targets including Bak, The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization, These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway, a repressor of human telomerase reverse transcriptase (hTERT), and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation, are degraded by E6/E6AP causing inhibition of apoptosis, Plays a major role in the induction and maintenance of cellular transformation |
| Purification method: |
Purified by Protein G |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
E6, Protein E6 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
HPV16 E6 protein (2E3) Monoclonal |
| Short name: |
HPV16 E6 protein (2E3) Monoclonal Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HPV16 E6 protein (2E3) monoclonal (Antibody to) |
| Alternative technique: |
antibodies |