| Long name: |
Biotin Conjugated, PHD2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PHD2 |
| Specificity: |
This is a highly specific antibody against PHD2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-70/426 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human PHD2 |
| Gene ID number: |
54583 |
| Swiss Prot: |
Q9GZT9 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also hydroxylates HIF2A, CODD) of HIF1A, EGLN1 is the most important isozyme under normoxia and, Has a preference for the CODD site for both HIF1A and HIF1B, Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex, Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, Target proteins are preferencially recognized via a LXXLAP motif, Under hypoxic conditions, and C-terminal, and increased expression of hypoxy-inducible genes, heterodimerization with HIF1B, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, through regulating the stability of HIF1, under normoxic conditions, Cellular oxygen sensor that catalyzes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
SM-20 PHD2 ZMYND6 HIFPH2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PHD2 Conjugated |
| Short name: |
Conjugated, PHD2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, PHD2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1232 |
| Gene: |
EGLN1 |
More about : EGLN1 |
| Long gene name: |
egl-9 family hypoxia inducible factor 1 |
| Synonyms gene: |
C1orf12 |
| Synonyms gene name: |
EGL nine (C, elegans) egl-9 family hypoxia-inducible factor 1 , elegans) homolog 1 egl nine homolog 1 (C |
| Synonyms name: |
HIF prolyl hydroxylase 2 |
| Locus: |
1q42, 2 |
| Discovery year: |
2000-06-16 |
| GenBank acession: |
AJ310543 |
| Entrez gene record: |
54583 |
| Pubmed identfication: |
11056053 |
| RefSeq identity: |
NM_022051 |
| Classification: |
Zinc fingers MYND-type |
| Havana BLAST/BLAT: |
OTTHUMG00000038027 |