| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PHD2 |
| Specificity: |
This antibody reacts specifically with PHD2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PHD2 |
| Gene ID: |
54583 |
| Swiss Prot: |
Q9GZT9 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also hydroxylates HIF2A, CODD) of HIF1A, EGLN1 is the most important isozyme under normoxia and, Has a preference for the CODD site for both HIF1A and HIF1B, Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex, Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, Target proteins are preferencially recognized via a LXXLAP motif, Under hypoxic conditions, and C-terminal, and increased expression of hypoxy-inducible genes, heterodimerization with HIF1B, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, through regulating the stability of HIF1, under normoxic conditions, Cellular oxygen sensor that catalyzes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SM-20 PHD2 ZMYND6 HIFPH2 |
| Also known as: |
PHD2 Polyclonal Antibody |
| Other name: |
Anti-PHD2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PHD2 |
| Short name: |
Anti-PHD2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PHD2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1232 |
| Gene: |
EGLN1 |
More about : EGLN1 |
| Long gene name: |
egl-9 family hypoxia inducible factor 1 |
| Synonyms gene: |
C1orf12 |
| Synonyms gene name: |
EGL nine (C, elegans) egl-9 family hypoxia-inducible factor 1 , elegans) homolog 1 egl nine homolog 1 (C |
| Synonyms name: |
HIF prolyl hydroxylase 2 |
| Locus: |
1q42, 2 |
| Discovery year: |
2000-06-16 |
| GenBank acession: |
AJ310543 |
| Entrez gene record: |
54583 |
| Pubmed identfication: |
11056053 |
| RefSeq identity: |
NM_022051 |
| Classification: |
Zinc fingers MYND-type |
| Havana BLAST/BLAT: |
OTTHUMG00000038027 |