| Long name: |
FITC Conjugated, PHD2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PHD2 |
| Specificity: |
This is a highly specific antibody against PHD2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-70/426 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human PHD2 |
| Gene ID number: |
54583 |
| Swiss Prot: |
Q9GZT9 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also hydroxylates HIF2A, CODD) of HIF1A, EGLN1 is the most important isozyme under normoxia and, Has a preference for the CODD site for both HIF1A and HIF1B, Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex, Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, Target proteins are preferencially recognized via a LXXLAP motif, Under hypoxic conditions, and C-terminal, and increased expression of hypoxy-inducible genes, heterodimerization with HIF1B, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, through regulating the stability of HIF1, under normoxic conditions, Cellular oxygen sensor that catalyzes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SM-20 PHD2 ZMYND6 HIFPH2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PHD2 Conjugated |
| Short name: |
FITC Conjugated, PHD2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PHD2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1232 |
| Gene: |
EGLN1 |
More about : EGLN1 |
| Long gene name: |
egl-9 family hypoxia inducible factor 1 |
| Synonyms gene: |
C1orf12 |
| Synonyms gene name: |
EGL nine (C, elegans) egl-9 family hypoxia-inducible factor 1 , elegans) homolog 1 egl nine homolog 1 (C |
| Synonyms name: |
HIF prolyl hydroxylase 2 |
| Locus: |
1q42, 2 |
| Discovery year: |
2000-06-16 |
| GenBank acession: |
AJ310543 |
| Entrez gene record: |
54583 |
| Pubmed identfication: |
11056053 |
| RefSeq identity: |
NM_022051 |
| Classification: |
Zinc fingers MYND-type |
| Havana BLAST/BLAT: |
OTTHUMG00000038027 |