| Long name: |
ALEXA FLUOR 647 Conjugated, Smad2(Ser465 + Ser467) Antibody |
| Also known as: |
Anti-Smad2 Ser465 + Ser467 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Smad2 Ser465 + Ser467 |
| Specificity: |
This is a highly specific antibody against Smad2 Ser465 + Ser467 |
| Modification: |
Phosphorylation |
| Modification Site: |
Ser465 + Ser467 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467 |
| Gene ID Number: |
4087 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
(Smad4) that functions via its interaction to the various Smad's, (Smad6 and Smad7), 5, Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, Smad2 consists of two highly conserved domains, The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors, The Smad family is divided into three subclasses: receptor-regulated Smad's, The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling, This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, allowing its binding to DNA and transcription factors, and 8), and the inhibitory Smad's, differentiation and development, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains, the common partner, which is negatively regulated by calmodulin, Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
JV18, JV18 1, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, SMAD, SMAD 2, SMAD2, SMAD2_HUMAN, Smad2p-S465/467, hMAD 2, hSMAD2, mothers against DPP homolog 2, p-Smad2Ser465/467, Smad2p-Ser465/467 |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the Smad2(Ser465 + Ser467) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
Smad2(Ser465 + Ser467) |
| Short name: |
Smad2(Ser465 + Ser467) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, SMAD family member 2(Ser465 and Ser467) (Antibody to) |
| Alternative technique: |
antibodies |