| Long name: |
Biotin Conjugated, Smad2(Ser465 + Ser467) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Smad2 Ser465 + Ser467 |
| Specificity: |
This is a highly specific antibody against Smad2 Ser465 + Ser467 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser465 + Ser467 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467 |
| Gene ID number: |
4087 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
(Smad4) that functions via its interaction to the various Smad's, (Smad6 and Smad7), 5, Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, Smad2 consists of two highly conserved domains, The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors, The Smad family is divided into three subclasses: receptor-regulated Smad's, The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling, This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, allowing its binding to DNA and transcription factors, and 8), and the inhibitory Smad's, differentiation and development, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains, the common partner, which is negatively regulated by calmodulin, Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
JV18, JV18 1, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, SMAD, SMAD 2, SMAD2, SMAD2_HUMAN, Smad2p-S465/467, hMAD 2, hSMAD2, mothers against DPP homolog 2, p-Smad2Ser465/467, Smad2p-Ser465/467 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Smad2(Ser465 + Ser467) Conjugated |
| Short name: |
Conjugated, Smad2(Ser465 + Ser467) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, SMAD family member 2(Ser465 and Ser467) (Antibody to) |
| Alternative technique: |
antibodies |