| Alternative name 1: |
Clone: 15F, FITC, Rat IgMk, Rat Mab anti- Mouse, 10E5, CD226 |
| Alternative name 2: |
Clone: 15F, FITC, Rat IgMk, Rat Mab antibody- Mouse, 10E5, CD226 |
| Alternative name 3: |
Clone: 15F, FITC, Rattus norvegicus IgMk, Rattus norvegicus Mab antibody- Mus musculus, 10E5, CD226 |
| Alternative name 4: |
10E5 IgMk, anti-CD226 Clone: 15F |
| Alternative name 5: |
10E5 IgMk, CD226 Clone: 15F |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , There are less rat- than mouse clones however, Monoclonals of this antigen are available in different clones, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Rattus norvegicus, Mus musculus |
| French translation: |
anticorps |
| Gene target: |
IgMk, Monoclonal 15F, 10E5, CD226 |
| Short name: |
Clone: 15F, FITC, IgMk, Mouse Monoclonal antibody- Mouse, 10E5, CD226 |
| Technique: |
Fluorescein, Monoclonals or monoclonal antibodies, mouses, Mouse |
| Host: |
Rat |
| Label: |
FITC |
| Species: |
Mouses, Rats, Rat |
| Alternative name: |
Rat IgMk, Rat Mouse monoclonal (Antibody to)- Mouse, clonality: 15F, fluorecein, 10E5, CD226 molecule |
| Alternative technique: |
murine |
| Alternative to gene target: |
CD226 and IDBG-4779 and ENSG00000150637 and 10666, CD226 and IDBG-643547 and ENSBTAG00000009266 and 615496, Cd226 and IDBG-163478 and ENSMUSG00000034028 and 225825, Cell surfaces, DNAM-1 and DNAM1 and PTA1 and TLiSA1, cell adhesion molecule binding, this GO :0001816 and cytokine production and biological process this GO :0002860 and positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target and biological process this GO :0002891 and positive regulation of immunoglobulin mediated immune response and biological process this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0008037 and cell recognition and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0019901 and protein kinase binding and molecular function this GO :0033005 and positive regulation of mast cell activation and biological process this GO :0045121 and membrane raft and cellular component this GO :0045954 and positive regulation of natural killer cell mediated cytotoxicity and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050839 and cell adhesion molecule binding and molecular function this GO :0060369 and positive regulation of Fc receptor mediated stimulatory signaling pathway and biological process, this GO :0005178 : integrin binding, this GO :0005178 : integrin binding and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding and also this GO :0050839 : cell adhesion molecule binding, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, this GO :0050839 : cell adhesion molecule binding, CD226 molecule |
| Identity: |
16961 |
| Gene: |
CD226 |
More about : CD226 |
| Long gene name: |
CD226 molecule |
| Synonyms gene name: |
CD226 antigen |
| Synonyms: |
DNAM-1 DNAM1 PTA1 TLiSA1 |
| Locus: |
18q22, 2 |
| Discovery year: |
2003-10-02 |
| GenBank acession: |
U56102 |
| Entrez gene record: |
10666 |
| Pubmed identfication: |
8673704 |
| RefSeq identity: |
NM_006566 |
| Classification: |
CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132809 |