| Long name: |
FITC Conjugated, SLP76 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SLP76 |
| Specificity: |
This is a highly specific antibody against SLP76 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human SLP76 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, However, Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production, The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response, Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR), When co-expressed, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1, serine/threonine or lipid kinase families, the biochemical basis of Vavâ, &euro, &trade, The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation, s function is unclear |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SLP-76 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
SLP76 Conjugated |
| Short name: |
FITC Conjugated, SLP76 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SLP76 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6529 |
| Gene: |
LCP2 |
More about : LCP2 |
| Long gene name: |
lymphocyte cytosolic protein 2 |
| Synonyms gene: |
SLP76 |
| Synonyms gene name: |
lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD) lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) |
| Synonyms name: |
76 kDa tyrosine phosphoprotein SH2 domain-containing leukocyte protein of 76kD |
| Locus: |
5q35, 1 |
| Discovery year: |
1996-03-12 |
| Entrez gene record: |
3937 |
| Pubmed identfication: |
7706237 |
| RefSeq identity: |
NM_005565 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000163121 |