| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SLP76 |
| Specificity: |
This antibody reacts specifically with SLP76 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SLP76 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, However, Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production, The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response, Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR), When co-expressed, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1, serine/threonine or lipid kinase families, the biochemical basis of Vavâ, &euro, &trade, The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation, s function is unclear |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SLP-76 |
| Also known as: |
SLP76 Polyclonal Antibody |
| Other name: |
Anti-SLP76 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SLP76 |
| Short name: |
Anti-SLP76 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SLP76 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6529 |
| Gene: |
LCP2 |
More about : LCP2 |
| Long gene name: |
lymphocyte cytosolic protein 2 |
| Synonyms gene: |
SLP76 |
| Synonyms gene name: |
lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD) lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) |
| Synonyms name: |
76 kDa tyrosine phosphoprotein SH2 domain-containing leukocyte protein of 76kD |
| Locus: |
5q35, 1 |
| Discovery year: |
1996-03-12 |
| Entrez gene record: |
3937 |
| Pubmed identfication: |
7706237 |
| RefSeq identity: |
NM_005565 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000163121 |