| Long name: |
FITC Conjugated, ASAH1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ASAH1 |
| Specificity: |
This is a highly specific antibody against ASAH1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Acid ceramidase subunit beta |
| Gene ID number: |
427 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Bioinformatic analysis of gene expression also reveals acid ceramidase to be among the 5 most important genes associated with melanoma, Effected individuals experience early onset joint problems and neurological problems, In addition to ceramide hydrolysis, Interestingly, a hallmark of Farber disease, and deficiency leads to accumulation of ceramide in tissues, for hydrolysis the pH optimum is 4, further supporting a complex and central role for acid ceramidase in sphingolipid metabolism, owing to mutations in the acid ceramidase gene, pH regulates which reaction is favored, purified acid ceramidase also exhibits the ability to catalyze ceramide synthesis, utilizing [14C]lauric acid and sphingosine as substrates, whereas for the reverse reaction favors a pH of 5, 5, 5, Acid ceramidase catalyzes the degradation of ceramide in normal tissues |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AC PHP32 FLJ21558 ACDase |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ASAH1 Conjugated |
| Short name: |
FITC Conjugated, ASAH1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ASAH1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
735 |
| Gene: |
ASAH1 |
More about : ASAH1 |
| Long gene name: |
N-acylsphingosine amidohydrolase 1 |
| Synonyms gene: |
ASAH |
| Synonyms gene name: |
N-acylsphingosine amidohydrolase (acid ceramidase) |
| Synonyms name: |
acylsphingosine deacylase acid ceramidase |
| Locus: |
8p22 |
| Discovery year: |
1997-08-22 |
| GenBank acession: |
U70063 |
| Entrez gene record: |
427 |
| Pubmed identfication: |
8955159 10610716 |
| RefSeq identity: |
NM_004315 |
| Havana BLAST/BLAT: |
OTTHUMG00000096997 |