| Long name: |
FITC Conjugated, ARP2/ARP3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ARP2/ARP3 |
| Specificity: |
This is a highly specific antibody against ARP2/ARP3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ARP2/ARP3 |
| Gene ID number: |
10097 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, In addition, In response to GTP-binding Cdc42, The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, and initiates the formation of lamellipodia and filopodia, and nucleates growth in the barbed direction, and platelet activation, and various other accessory proteins, cell adhesion, cell migration, including chemotaxis, namely the WASP proteins, or by associating with the side of an existing filament, or the uncapping of existing barbed ends, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases, the Arp2/3 complex binds the Cdc42 substrates, the severing of existing filaments to create uncapped barbed ends, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork, Actin polymerization is required for a variety of cell functions |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ARP2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ARP2/ARP3 Conjugated |
| Short name: |
FITC Conjugated, ARP2/ARP3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ARP2/ARP3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
169 |
| Gene: |
ACTR2 |
More about : ACTR2 |
| Long gene name: |
ARP2 actin related protein 2 homolog |
| Synonyms gene name: |
ARP2 (actin-related protein 2, yeast) homolog ARP2 actin-related protein 2 homolog (yeast) |
| Locus: |
2p14 |
| Discovery year: |
1999-05-17 |
| GenBank acession: |
AF006082 |
| Entrez gene record: |
10097 |
| Pubmed identfication: |
9230079 |
| RefSeq identity: |
NM_001005386 |
| Classification: |
Actin related protein 2/3 complex |
| Havana BLAST/BLAT: |
OTTHUMG00000129540 |