| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ARP2/ARP3 |
| Specificity: |
This antibody reacts specifically with ARP2/ARP3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ARP2/ARP3 |
| Gene ID: |
10097 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, In addition, In response to GTP-binding Cdc42, The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, and initiates the formation of lamellipodia and filopodia, and nucleates growth in the barbed direction, and platelet activation, and various other accessory proteins, cell adhesion, cell migration, including chemotaxis, namely the WASP proteins, or by associating with the side of an existing filament, or the uncapping of existing barbed ends, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases, the Arp2/3 complex binds the Cdc42 substrates, the severing of existing filaments to create uncapped barbed ends, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork, Actin polymerization is required for a variety of cell functions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ARP2 |
| Also known as: |
ARP2/ARP3 Polyclonal Antibody |
| Other name: |
Anti-ARP2/ARP3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ARP2/ARP3 |
| Short name: |
Anti-ARP2/ARP3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ARP2/ARP3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
169 |
| Gene: |
ACTR2 |
More about : ACTR2 |
| Long gene name: |
ARP2 actin related protein 2 homolog |
| Synonyms gene name: |
ARP2 (actin-related protein 2, yeast) homolog ARP2 actin-related protein 2 homolog (yeast) |
| Locus: |
2p14 |
| Discovery year: |
1999-05-17 |
| GenBank acession: |
AF006082 |
| Entrez gene record: |
10097 |
| Pubmed identfication: |
9230079 |
| RefSeq identity: |
NM_001005386 |
| Classification: |
Actin related protein 2/3 complex |
| Havana BLAST/BLAT: |
OTTHUMG00000129540 |