| Long name: |
HRP Conjugated, APC10 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
HRP Conjugated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
APC10 |
| Specificity: |
This is a highly specific antibody against APC10 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human APC10 |
| Gene ID number: |
10393 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
APC, APC is phosphorylated, APC is under tight control by a number of regulatory factors, APC10 also localizes to kinetochores from prophase to anaphase, APC10 binds to core APC subunits throughout the cell cycle, APC10 binds to the C-terminus of CDC27/APC3, APC10 contains a Doc1 homology domain, APC10 is localized in centrosomes and mitotic spindles, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs, CDH1 and MAD2, During mitosis, In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, Specifically, Specifically, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division, and thus activated, and to the midbody in telophase and cytokinesis, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk), including CDC20, or cyclosome, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis, thus preventing APC activation, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases, Composed of more than ten subunits |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% Gentamicin, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
APC10 DOC1 DKFZP564L0562 |
| Properties: |
Also monoclonal HRP conjugates are often used for Western Blot, HRP conjugates are often primary rabbit polyclonal antibodies couples to the , found in the roots of , (HRP), C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°, enzyme , horseradish, horseradish peroxidase |
| Gene target: |
APC10 Conjugated |
| Short name: |
Conjugated, APC10 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
HRP |
| Alternative name: |
horseradish peroxidase coupled, APC10 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
24077 |
| Gene: |
ANAPC10 |
More about : ANAPC10 |
| Long gene name: |
anaphase promoting complex subunit 10 |
| Locus: |
4q31, 21 |
| Discovery year: |
2004-05-21 |
| GenBank acession: |
AF132794 |
| Entrez gene record: |
10393 |
| Pubmed identfication: |
10318877 11230166 |
| RefSeq identity: |
NM_014885 |
| Classification: |
Anaphase promoting complex |
| Havana BLAST/BLAT: |
OTTHUMG00000161477 |