| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
APC10 |
| Specificity: |
This antibody reacts specifically with APC10 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human APC10 |
| Gene ID: |
10393 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
APC, APC is phosphorylated, APC is under tight control by a number of regulatory factors, APC10 also localizes to kinetochores from prophase to anaphase, APC10 binds to core APC subunits throughout the cell cycle, APC10 binds to the C-terminus of CDC27/APC3, APC10 contains a Doc1 homology domain, APC10 is localized in centrosomes and mitotic spindles, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs, CDH1 and MAD2, During mitosis, In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, Specifically, Specifically, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division, and thus activated, and to the midbody in telophase and cytokinesis, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk), including CDC20, or cyclosome, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis, thus preventing APC activation, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases, Composed of more than ten subunits |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
APC10 DOC1 DKFZP564L0562 |
| Also known as: |
APC10 Polyclonal Antibody |
| Other name: |
Anti-APC10 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
APC10 |
| Short name: |
Anti-APC10 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-APC10 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
24077 |
| Gene: |
ANAPC10 |
More about : ANAPC10 |
| Long gene name: |
anaphase promoting complex subunit 10 |
| Locus: |
4q31, 21 |
| Discovery year: |
2004-05-21 |
| GenBank acession: |
AF132794 |
| Entrez gene record: |
10393 |
| Pubmed identfication: |
10318877 11230166 |
| RefSeq identity: |
NM_014885 |
| Classification: |
Anaphase promoting complex |
| Havana BLAST/BLAT: |
OTTHUMG00000161477 |