| Long name: |
Biotin Conjugated, PBEF1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PBEF1 |
| Specificity: |
This is a highly specific antibody against PBEF1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
290-340/491 |
| Subcellular location: |
Nucleus, Secreted, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human PBEF |
| Gene ID number: |
10135 |
| Swiss Prot: |
P43490 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It is the rate limiting component in the mammalian NAD biosynthesis pathway, NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity), Plays a role in the modulation of circadian clock function, The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, an intermediate in the biosynthesis of NAD, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma, Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
PBEF |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PBEF1 Conjugated |
| Short name: |
Conjugated, PBEF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, PBEF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
30092 |
| Gene: |
NAMPT |
More about : NAMPT |
| Long gene name: |
nicotinamide phosphoribosyltransferase |
| Synonyms gene: |
PBEF1 |
| Synonyms gene name: |
pre-B-cell colony enhancing factor 1 |
| Synonyms name: |
visfatin |
| Locus: |
7q22, 3 |
| Discovery year: |
2004-02-02 |
| GenBank acession: |
U02020 |
| Entrez gene record: |
10135 |
| Pubmed identfication: |
8289818 |
| RefSeq identity: |
NM_182790 |
| Havana BLAST/BLAT: |
OTTHUMG00000140388 |