| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Anti-LGR5 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the anti-LGR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This anti-LGR5 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O75473 |
| Purity: |
Purified |
| Description: |
In contrast to classical G-protein coupled receptors, Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development, RSPO2, RSPO3 or RSPO4), Upon binding to R-spondins (RSPO1, [UniProt], associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, does not activate heterotrimeric G-proteins to transduce the signal, triggering the canonical Wnt signaling pathway to increase expression of target genes, LGR5/GPR49 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle |
| Immunogen: |
A portion of amino acids 605-638 from the human protein was used as the immunogen for this anti-LGR5 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-LGR5 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
LGR5 |
| Short name: |
Anti-LGR5 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
antibody to-LGR5 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4504 |
| Gene: |
LGR5 |
More about : LGR5 |
| Long gene name: |
leucine rich repeat containing G protein-coupled receptor 5 |
| Synonyms gene: |
GPR67 GPR49 |
| Synonyms gene name: |
G protein-coupled receptor 49 |
| Synonyms: |
HG38 FEX |
| Locus: |
12q21, 1 |
| Discovery year: |
1999-10-22 |
| GenBank acession: |
AF062006 |
| Entrez gene record: |
8549 |
| Pubmed identfication: |
9642114 |
| RefSeq identity: |
NM_003667 |
| Classification: |
Class A orphans , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000169543 |