| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PKR (PRKR) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This PKR antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P19525 |
| Purity: |
Purified |
| Description: |
Activation by dsRNAs leads to autophosphorylation of PKR and allows the kinase to phosphorylate its natural substrate, Apoptosis induced by live pathogenic gram-positive and gram-negative bacteria requirs both TLR4 and PKR, PRKR, activates IKK and NFKB, double-stranded RNA-activated protein kinase (PRKR/PKR) is a serine-threonine kinase, leading to the inhibition of protein synthesis, possibly representing a major mechanism for pathogenic bacteria that use specific virulence factors to avoid detection and destruction by the innate immune system, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), thereby suppressing apoptosis, which is involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, Interferon-induced |
| Immunogen: |
A portion of amino acids 11-42 from the human protein was used as the immunogen for this PKR antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PKR antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PKR (PRKR) |
| Short name: |
PKR Antibody (PRKR) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PKR (Antibody to) (PRKR) |
| Alternative technique: |
antibodies |
| Identity: |
9437 |
| Gene: |
EIF2AK2 |
More about : EIF2AK2 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 2 |
| Synonyms gene: |
PRKR |
| Synonyms gene name: |
interferon-inducible double stranded RNA dependent , protein kinase |
| Synonyms: |
PKR EIF2AK1 PPP1R83 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 83 |
| Locus: |
2p22, 2 |
| Discovery year: |
1992-12-04 |
| GenBank acession: |
BC057805 |
| Entrez gene record: |
5610 |
| Pubmed identfication: |
1351683 |
| RefSeq identity: |
NM_002759 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000100962 |