| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PKR (PRKR) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PKR antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P19525 |
| Purity: |
Antigen affinity |
| Description: |
1996), Activation of the protein allows the kinase to phosphorylate its natural substrate, Ben-Asouli et al, By FISH analysis, E2 inhibited the kinase activity of the protein and blocked its inhibitory effect on protein synthesis and cell growth, IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region, Squire et al, Taylor et al, They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and its target, also called PKR (Protein kinase RNA activated), is an enzyme that in humans is encoded by the EIF2AK2 gene, leading to the inhibition of protein synthesis(summary by Kuhen et al, the alpha subunit of eukaryotic protein synthesis initiation factor-2, the translation initiation factor EIF2-alpha, (1993) assigned the gene to the boundary between chromosome 2p22-p21, (1999) studied the mechanism underlying the resistance of hepatitis C virus(HCV) to interferon, (2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield, Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2 |
| Immunogen: |
An amino acid sequence from the N-terminus of human Protein kinase RNA activated (AGDLSAGFFMEELNTYR) was used as the immunogen for this PKR antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PKR antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PKR (PRKR) |
| Short name: |
PKR Antibody (PRKR) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PKR (Antibody to) (PRKR) |
| Alternative technique: |
antibodies |
| Identity: |
9437 |
| Gene: |
EIF2AK2 |
More about : EIF2AK2 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 2 |
| Synonyms gene: |
PRKR |
| Synonyms gene name: |
interferon-inducible double stranded RNA dependent , protein kinase |
| Synonyms: |
PKR EIF2AK1 PPP1R83 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 83 |
| Locus: |
2p22, 2 |
| Discovery year: |
1992-12-04 |
| GenBank acession: |
BC057805 |
| Entrez gene record: |
5610 |
| Pubmed identfication: |
1351683 |
| RefSeq identity: |
NM_002759 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000100962 |