| Gene info: |
The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204, Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory |
| Gene names: |
ERT1, Erk2, MAP kinase 1, Mapk, Prkm1, Erk2 |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Spicific to: |
ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 |
| Antigen: |
ERK/MAPK (Thr202/Tyr204) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK |
| Immuno application: |
immuno-histo-chemistry, Western Blot |
| Antibody advice: |
Applications include Dot Blots (DB) and Western Blots (WB), Dot blots and Western blots with a rat brain lysate were performed with each lot, Human, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody, Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stock solutions are stable for a minimum of 1 year at -20 grades C, but shorter term storage at -20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, For long term storage -80 grades C is recommended |
| Works with: |
Mouse, Rat, Xenopus, Human |
| Omim nr: |
52001075 |
| Ncbi nr: |
P63086 |
| KDa: |
42/44 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
116590 |
| GENE symbol: |
Mapk1 |
| Antigene name: |
mitogen activated protein kinase 1 |
| Source: |
Rattus norvegicus |
| Protein nr: |
P63086 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
ERK1 / 2 (phospho Thr202 / Tyr204) |
| Short name: |
anti-ERK1 / 2 (phospho Thr202 / Tyr204) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-ERK1 / 2 (phosphorilated Thr202 / Tyr204) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6877 |
| Gene: |
MAPK3 |
More about : MAPK3 |
| Long gene name: |
mitogen-activated protein kinase 3 |
| Synonyms gene: |
PRKM3 |
| Synonyms: |
ERK1 p44mapk p44erk1 |
| Locus: |
16p11, 2 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84490 |
| Entrez gene record: |
5595 |
| Pubmed identfication: |
9628824 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000132149 |