| Long name: |
ALEXA FLUOR 488 Conjugated, ERK1/2(Thr202 + Tyr204) Antibody |
| Also known as: |
Anti-ERK1/2 Thr202 + Tyr204 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ERK1/2 Thr202 + Tyr204 |
| Specificity: |
This is a highly specific antibody against ERK1/2 Thr202 + Tyr204 |
| Modification: |
Phosphorylation |
| Modification Site: |
Thr202 + Tyr204 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
196-210/379 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human p44/42 MAPK around the phosphorylation site of (Thr202/Tyr204) |
| Gene ID Number: |
5595 |
| Swiss Prot: |
P27361 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Bovine, Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Cell stimulation induces the activation of a signaling cascade, ERK (extracellular signal regulated kinase), ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, and bioactive peptides, cytokines, growth factors, hormones, respectively, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton, thereby greatly elevating the activity of ERK1&, 2, p44/42 MAP Kinase(Thr202) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
ERK1 p44mapk p44erk1 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the ERK1/2(Thr202 + Tyr204) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
ERK1/2(Thr202 + Tyr204) |
| Short name: |
ERK1/2(Thr202 + Tyr204) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, ERK1/2(Thr202 and Tyr204) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6877 |
| Gene: |
MAPK3 |
More about : MAPK3 |
| Long gene name: |
mitogen-activated protein kinase 3 |
| Synonyms gene: |
PRKM3 |
| Locus: |
16p11, 2 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84490 |
| Entrez gene record: |
5595 |
| Pubmed identfication: |
9628824 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000132149 |