| Category: |
Antibody |
| Concentration: |
05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 2 mg/ml in 1X PBS with 0 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
AMACR |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
, Due to limited knowledge and inability to test the antibody against all known species, Other species not tested, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
Immunohistology (FFPE): 1:50-1:100 for 30-60 minutes at RT (1), Prediluted format : incubate for 30 min at RT (2) |
| Notes: |
After epitope retrieval step (if required), Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, The prediluted format is supplied in a dropper bottle and is optimized for use in IHC, drip mAb solution onto the tissue section and incubate at RT for 30 min, for 10-20 min followed by cooling at RT for 20 minutes 2, pH 9, <, 1, Optimal dilution of the AMACR antibody should be determined by the researcher, p> |
| Intented use: |
This AMACR antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UHK6 |
| Purity: |
Purified by protein A Affinity Chromatography |
| Description: |
A spotty granular cytoplasmic staining is seen in a few cells of the benign glands, AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma, AMACR is essential in lipid metabolism, AMACR is expressed in normal liver (hepatocytes), AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia, Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium), It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids, It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma, Past studies have also shown that AMACR is expressed in various colon carcinomas (well, The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction, also known as p504S, kidney (tubular epithelial cells) and gall bladder (epithelial cells), moderately and poorly differentiated) and over expressed in prostate carcinoma, which is identified as AMACR, This antibody recognizes a protein of 54kDa |
| Immunogen: |
A synthetic peptide from the human protein was used as the immunogen for the AMACR antibody |
| Storage: |
Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide) |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
AMACR |
| Short name: |
AMACR Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
AMACR (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
451 |
| Gene: |
AMACR |
More about : AMACR |
| Long gene name: |
alpha-methylacyl-CoA racemase |
| Synonyms: |
RACE P504S |
| Locus: |
5p13, 2 |
| Discovery year: |
1999-10-19 |
| GenBank acession: |
AF047020 |
| Entrez gene record: |
23600 |
| Pubmed identfication: |
9307041 |
| RefSeq identity: |
NM_014324 |
| Havana BLAST/BLAT: |
OTTHUMG00000090734 |