| Long name: |
ALEXA FLUOR 647 Conjugated, AMPK gamma 3/PRKAG3 Polyclonal Antibody |
| Also known as: |
Anti-AMPK gamma 3/PRKAG3 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK gamma 3/PRKAG3 |
| Specificity: |
This is a highly specific antibody against AMPK gamma 3/PRKAG3 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
174-220/489 |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human AMPK gamma 3 |
| Gene ID Number: |
53632 |
| Swiss Prot: |
Q9UGI9 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
ADP also stimulates phosphorylation, ADP and ATP, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, ATP promotes dephosphorylation of catalytic subunit, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Gamma non-catalytic subunit mediates binding to AMP, In response to reduction of intracellular ATP levels, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits, probably by indirectly activating myosin, rendering the AMPK enzyme inactive, without stimulating already phosphorylated catalytic subunit, AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the AMPK gamma 3/PRKAG3 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
AMPK gamma 3/PRKAG3 |
| Short name: |
AMPK gamma 3/PRKAG3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, AMP-activated, g 3 non-catalytic subunit (Antibody to), AMPK g 3/protein phosphorylation catalyst |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AMP-activated, Extracellular, PRKAG3 and IDBG-643891 and ENSBTAG00000013492 and 511961, PRKAG3 and IDBG-81160 and ENSG00000115592 and 53632, Prkag3 and IDBG-168633 and ENSMUSG00000006542 and 241113, adenyl nucleotide binding, gamma 3 non-catalytic subunit, this GO :0004679 and AMP-activated protein kinase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005978 and glycogen biosynthetic process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006633 and fatty acid biosynthetic process and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0015758 and glucose transport and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030554 and adenyl nucleotide binding and molecular function this GO :0031588 and AMP-activated protein kinase complex and cellular component this GO :0035556 and intracellular signal transduction and biological process this GO :0061024 and membrane organization and biological process, this GO :0004679 : AMP-activated protein kinase activity, this GO :0004679 : AMP-activated protein kinase activity and also this GO :0005524 : ATP binding and also this GO :0016301 : kinase activity and also this GO :0019901 : protein kinase binding and also this GO :0030554 : adenyl nucleotide binding, this GO :0005524 : ATP binding, this GO :0016301 : kinase activity, this GO :0019901 : protein kinase binding, this GO :0030554 : adenyl nucleotide binding, protein kinase |
| Identity: |
9387 |
| Gene: |
PRKAG3 |
More about : PRKAG3 |
| Long gene name: |
protein kinase AMP-activated non-catalytic subunit gamma 3 |
| Synonyms gene name: |
AMP-activated, gamma 3 non-catalytic subunit , protein kinase |
| Locus: |
2q35 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AF214519 |
| Entrez gene record: |
53632 |
| Pubmed identfication: |
10818001 |
| Havana BLAST/BLAT: |
OTTHUMG00000133078 |