| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DDX5 Tyr593 |
| Specificity: |
This antibody reacts specifically with DDX5 (Tyr593) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr593 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human DDX5 around the phosphorylation site of Tyr593 |
| Gene ID: |
1655 |
| Swiss Prot: |
P17844 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as transcriptional repressor in a promoter-specicic manner, As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms, Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10, Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity, Involved in transcriptional regulation, Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation, The rate of ATP hydrolysis is highly stimulated by single-stranded RNA, Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation, Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR, Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis, its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner, such as HDAC1, the function is independent of the RNA helicase activity, the function probbaly involves association with histone deacetylases, Involved in the alternative regulation of pre-mRNA splicing |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p68 |
| Also known as: |
DDX5 (Tyr593) Polyclonal Antibody |
| Other name: |
Anti-DDX5 (Tyr593) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DDX5 (Tyr593) |
| Short name: |
Anti-DDX5 (Tyr593) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-DDX5 (Tyr593) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2746 |
| Gene: |
DDX5 |
More about : DDX5 |
| Long gene name: |
DEAD-box helicase 5 |
| Synonyms gene: |
HLR1 G17P1 |
| Synonyms gene name: |
68kD) DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 , DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase |
| Locus: |
17q23, 3 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
AF015812 |
| Entrez gene record: |
1655 |
| Pubmed identfication: |
22156369 18698352 |
| RefSeq identity: |
NM_004396 |
| Classification: |
DEAD-box helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000178936 |