| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FTO |
| Specificity: |
This antibody reacts specifically with FTO |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Fat mass and obesity-associated protein |
| Gene ID: |
79068 |
| Swiss Prot: |
Q9C0B1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Contributes to the regulation of body size and body fat accumulation, Contributes to the regulation of the global metabolic rate, Has highest activity towards single-stranded RNA containing 3-methyluracil, Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine, Has no activity towards 1-methylguanine, Has no detectable activity towards double-stranded DNA, Requires molecular oxygen, Specifically demethylates N(6)-methyladenosine (m6A) RNA, alpha-ketoglutarate and iron, energy expenditure and energy homeostasis, followed by single-stranded DNA containing 3-methylthymine, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes, Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA1752 MGC5149 ALKBH9 |
| Also known as: |
FTO Polyclonal Antibody |
| Other name: |
Anti-FTO Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FTO |
| Short name: |
Anti-FTO (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-fat mass and obesity associated (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-N1-methyladenine dioxygenase activity, FTO and IDBG-31147 and ENSG00000140718 and 79068, FTO and IDBG-631892 and ENSBTAG00000012501 and 618622, Fto and IDBG-179938 and ENSMUSG00000055932 and 26383, nuclei, this GO :0001659 and temperature homeostasis and biological process this GO :0005634 and nucleus and cellular component this GO :0006307 and DNA dealkylation involved in DNA repair and biological process this GO :0008198 and ferrous iron binding and molecular function this GO :0010883 and regulation of lipid storage and biological process this GO :0016607 and nuclear speck and cellular component this GO :0035515 and oxidative RNA demethylase activity and molecular function this GO :0035516 and oxidative DNA demethylase activity and molecular function this GO :0035552 and oxidative single-stranded DNA demethylation and biological process this GO :0035553 and oxidative single-stranded RNA demethylation and biological process this GO :0040014 and regulation of multicellular organism growth and biological process this GO :0042245 and RNA repair and biological process this GO :0043734 and DNA-N1-methyladenine dioxygenase activity and molecular function this GO :0044065 and regulation of respiratory system process and biological process this GO :0060612 and adipose tissue development and biological process this GO :0070350 and regulation of white fat cell proliferation and biological process this GO :0070989 and oxidative demethylation and biological process this GO :0080111 and DNA demethylation and biological process, this GO :0008198 : ferrous iron binding, this GO :0008198 : ferrous iron binding and also this GO :0035515 : oxidative RNA demethylase activity and also this GO :0035516 : oxidative DNA demethylase activity and also this GO :0043734 : DNA-N1-methyladenine dioxygenase activity, this GO :0035515 : oxidative RNA demethylase activity, this GO :0035516 : oxidative DNA demethylase activity, this GO :0043734 : DNA-N1-methyladenine dioxygenase activity, fat mass and obesity associated |
| Identity: |
24678 |
| Gene: |
FTO |
More about : FTO |
| Long gene name: |
FTO, alpha-ketoglutarate dependent dioxygenase |
| Synonyms gene name: |
fat mass and obesity associated |
| Synonyms name: |
alkB homolog 9 alpha-ketoglutarate-dependent dioxygenase |
| Locus: |
16q12, 2 |
| Discovery year: |
2007-04-12 |
| GenBank acession: |
BC003583 |
| Entrez gene record: |
79068 |
| Pubmed identfication: |
17434869 17991826 22002720 |
| RefSeq identity: |
NM_001080432 |
| Classification: |
Alkylation repair homologs |
| Havana BLAST/BLAT: |
OTTHUMG00000158780 |